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In the present work, hollow fiber-based microextraction (HFM) was used for the extraction of fluoxetine, diazepam and caffeine in the presence of temazepam (internal standard, IS) from a biological fluid. The procedure was based on the attachment of the hollow fibers to the inner surfaces of the needle of a syringe. The presence of hollow fibers avoided the need for an external phase change medium (that is, water or ethanol). The extraction solvent was selected to form a biphasic mixture with the acceptor solution to facilitate extraction. Two sets of experiments were carried out. Firstly, a simultaneous extraction of three drugs from blood plasma was developed. From this phase of the work, the effects of several parameters including extraction solvent type, fiber wall thickness and number of fiber wall layer, sample pH and volume, extraction time and ionic strength were investigated. The optimum results were obtained using 75 μm wall-thick hollow fibers, and 0.9% polyethylene glycol 8000 as extraction solvent. The enrichment factor was in the order of 12-14 and a good enrichment factor of 20 was obtained for the analytes. Secondly, the proposed method was validated for the extraction and determination of fluoxetine, diazepam and caffeine in a spiked urine sample. The analysis was carried out by high performance liquid chromatography with diode array detection. The results were validated according to the International Conference on Harmonization guidelines, obtaining good results for selectivity, accuracy, precision, linearity, limits of detection and quantification. what happens to kids in the U.S. who don’t get meals at school? What do those children eat? What food is actually available for them?

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